Super resolution microscopy reveals DHA-dependent alterations in glioblastoma membrane remodelling and cell migration
نویسندگان
چکیده
FABP7 nanodomains on glioblastoma neural stem-like cell membranes are greatly reduced when cells cultured in medium supplemented with the omega-3 polyunsaturated fatty acid DHA. Glioblastoma migration is also under these conditions.
منابع مشابه
Super-resolution Microscopy Reveals Compartmentalization of Peroxisomal Membrane Proteins*
Membrane-associated events during peroxisomal protein import processes play an essential role in peroxisome functionality. Many details of these processes are not known due to missing spatial resolution of technologies capable of investigating peroxisomes directly in the cell. Here, we present the use of super-resolution optical stimulated emission depletion microscopy to investigate with sub-6...
متن کاملSuper-resolution optical microscopy for studying membrane structure and dynamics.
Investigation of cell membrane structure and dynamics requires high spatial and temporal resolution. The spatial resolution of conventional light microscopy is limited due to the diffraction of light. However, recent developments in microscopy enabled us to access the nano-scale regime spatially, thus to elucidate the nanoscopic structures in the cellular membranes. In this review, we will expl...
متن کاملSuper-resolution microscopy reveals decondensed chromatin structure at transcription sites
Remodeling of the local chromatin structure is essential for the regulation of gene expression. While a number of biochemical and bioimaging experiments suggest decondensed chromatin structures are associated with transcription, a direct visualization of DNA and transcriptionally active RNA polymerase II (RNA pol II) at super-resolution is still lacking. Here we investigate the structure of chr...
متن کاملQuantitative Multicolor Super-Resolution Microscopy Reveals Tetherin HIV-1 Interaction
Virus assembly and interaction with host-cell proteins occur at length scales below the diffraction limit of visible light. Novel super-resolution microscopy techniques achieve nanometer resolution of fluorescently labeled molecules. The cellular restriction factor tetherin (also known as CD317, BST-2 or HM1.24) inhibits the release of human immunodeficiency virus 1 (HIV-1) through direct incor...
متن کاملLight-induced cell damage in live-cell super-resolution microscopy
Super-resolution microscopy can unravel previously hidden details of cellular structures but requires high irradiation intensities to use the limited photon budget efficiently. Such high photon densities are likely to induce cellular damage in live-cell experiments. We applied single-molecule localization microscopy conditions and tested the influence of irradiation intensity, illumination-mode...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nanoscale
سال: 2021
ISSN: ['2040-3372', '2040-3364']
DOI: https://doi.org/10.1039/d1nr02128a